ATTRACTING STORED OCTOPUS SPERM

Olfaction: Attracting Both Sperm and the Nose

Odorant receptor genes are expressed not only in the nose but also in testes, where they have been hypothesized to play a role in sperm chemotaxis. New data demonstrate that human odorant receptor hOR 17-4 may play similar roles in both tissues, lending support to the idea that chemical attraction is important for reproduction.

An N-terminal partial sequence of the 13 kDa Pycnopodia helianthoides sperm chemoattractant 'startrak' possesses sperm-attracting activity.

Freshwater extracts of starfish ovaries were used to purify the sperm-attracting peptide 'startrak' from Pycnopodia helianthoides using hydrophobic interaction chromatography and DEAE-high-pressure liquid chromatography. Partially purified attractant had a molecular mass of 13 kDa, estimated from gel filtration and polyacrylamide gel electrophoresis results. The purified attractant was subjecte...

Sperm-attractant peptide influences the spermatozoa swimming behavior in internal fertilization in Octopus vulgaris.

Marine invertebrates exhibit both chemokinesis and chemotaxis phenomena, induced in most cases by the release of water-borne peptides or pheromones. In mollusks, several peptides released during egg-laying improve both male attraction and mating. Unlike other cephalopods, Octopus vulgaris adopts an indirect internal fertilization strategy. We here report on the identification and characterizati...

Cryopreservation of in situ cool stored buffalo (Bubalus bubalis) epididymal sperm

The objectives of this study were to investigate the effects of testis-epididymis cool storage on viability and progressive motility of buffalo epididymal sperm (EP) and to compare the influences of two basic semen extenders on post thaw EP viability and progressive motility. Abattoir collected buffalo testicles were allotted to three storage times (0 h: n = 10; 24 h: n = 10 and 48 h: n = 12). ...

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